Pacific Biosciences Sequel DNA Sequencing System

This was removed from a university lab where it was surplus to requirements.

The unit was booted up on the client's site. Unfortunately, the PSU was not supplied to us.

The system boots up but shows errors in relation to a smrt link server. There was no smrt link server on the client's network which is what caused this issue.

It is in very good cosmetic condition but has a dent on one of the panels.

Comes with:-

(Boxed) Solid State Cooling Systems

Model - UC180

PN - 10-180-G1-P20-R

SN - 8008262

No power supply to test.

Consumables:-

1 x Sealed Pack of TX1009 AlphaWipe Polyester Wipers 9" x 9" (75 Wipes)

1 x Sealed Pack of TX404 Absorbond Polyester Wipers 4" x 4" (300 Wipes)

2 x Bottles of Sequel Coolant - Dynalene PG 30% - Both expired 2018/19

21 x Pacific Biosciences 96 well Verification Plates

6 x Bio-Rad Hard Shell 96-Well Sample Plates

1 x Pacbio Sequel Pipet Tips v2 (sealed)

1 x Pacbio Sequel Pipet Tips (31 tips left)

2 x Boxes Sequel SMRT Cell Oil (5 left in one/6 left in other)

1 x Tecan Conductive 200ul 96pcs rack (no tips)

4 x Packs SMRT Cell Mechanical (1 sealed/3 opened)

4 x Packs Pacbio Tube Septa (10 per pack)

1 x Pack of 11 Pacbio 0.5ml Empty NFC Verification Tubes

1 x Thermal Block

1 x Pack TX759B CleanTips Swabs (100 Swabs, Sealed)

1 x Uline Kapton Tape

1 x Red Ethernet Cable

2 x Pacbio Sequel Sample Plate Adhesive Foil

Thermofisher Quant Studio 5 Real-Time PCR Machine

Inventory ID: DAM_QS5F

Condition: Used

Year: 2021

Vin/Serial No: 272528491

Full working order, sale is to due to this system being surplus to requirements

This Applied Biosystems 7500 Fast Real-Time PCR System with PC & Software was removed from a university lab where it was surplus to requirements.

The unit powers up and has been connected to the PC. It has been inspected by a service engineer who has re-certified it.

The PC is a Dell Optiplex PC with a monitor, keyboard & Mouse with Life Version 2.3 7500 software installed on it. The machine also has a fresh install of Windows 7.

The 7500 has passed the following function tests:

USB

CCD

Filter Wheel

Shutter

Lamp

Thermal Cycler

All tests passed.

The lamp condition is described as 'good'. It has 209 hours usage and the current is 5.597amps. It is reported as being replaced 06/24/2020.

The unit has not been calibrated as it requires an Applied Biosystems real-time PCR system spectral calibration kit

Fluidigm Biomark HD Genetic Analyser & IFC Controller HX with Accessories

This was removed from a university lab where it was surplus to requirements.

The units are all in good cosmetic condition with marks from use. Everything powers up. Starting up various programs did trigger the main unit to open its side door ready for a sample so it does appear that the PC and main unit are communicating. We have not run samples on the instrument but all tests and software appear to be running as expected.

Lot contains:

1 x Fluidigm Biomark HD Genetic Analyser.

1 x Fluidigm IFC Controller HX.

1 x Dell Monitor.

1 x VGA Cable.

1 x Logitech Keyboard.

1 x USB Male to USB Female Cable.

1 x 9 Pin Male to 9 Pin Female Serial Cable (Not VGA).

We logged into the IFC Controller HX successfully.

Settings on controller say:

Serial No: HX-10641.

Chips Supported: 136, 138, 139, 153, 154.

Filmware Version: 53

Software Version: 2.7-2014-12-02.

Last System Check: 19/11/2021.

The PC built inside the Biomark HD Genetic Analyser has had the user data removed but the software remains

Fluidigm Software:

Genetic Analysis.

BioMarkICP

EP1

BioMark.

This Stratagene MX3005P Multiplex Quantitative PCR System was removed from a university lab where it was surplus to requirements.

The unit has been tested by a service engineer who has deemed it to be fully working.

The main unit powers up and is in good used cosmetic condition but it does have some scrapes, scratches and marks from use.

The system includes:

1 x Agilent Stratagene MX3005P. (various marks from use)

1 x Dell Optiplex 7010 with keyboard, Mouse & Monitor.

The PC is running a fresh install of Windows 7. Also installed is a copy of Stratagene MxPro - Mx3005P v4.10 build 389. The software will control the Mx3005P unit. We have run a basic heat cycle with it and verified that the lamp will turn on & off.

The MX3005P has the following filters installed:

1. CY5 - CY5 Filter Set - Excitation Wavelength(NM) 635 - Emission Wavelength(NM) 665.
2. CY3 - CY3 Filter Set - Excitation Wavelength(NM) 545 - Emission Wavelength(NM) 568.
3. ROX - ROX Filter Set - Excitation Wavelength(NM) 585 - Emission Wavelength(NM) 610
4. HEX - HEX-JOE Filter Set - Excitation Wavelength(NM) 535 - Emission Wavelength(NM) 555
5. FAM - FAM Filter Set - Excitation Wavelength(NM) 492 - Emission Wavelength(NM) 516

This Bio-Rad C1000 Thermal Cycler was removed from a university lab where it was surplus to requirements.

The item is in good cosmetic condition but there are some minor marks from prior use. There are no scratches or dents.

The item powered on without issue. It ran several self-tests and found no errors. The lid heating function works.

When set to 37˚C the thermometer measured 37.3˚C

When set to 100˚C the thermometer measured 98.2˚C

Affymetrix GeneChip Scanner 3000 System with Hybridization Ovens & Autoloader

This was removed from a university lab where it was surplus to requirements.

The system powers up and the PC has the software installed. It connected to the system, and we were able to control the scanner from the PC.

Included are;

Affymetrix GeneChip Scanner & Autoloader

Top:-

Model - Autoloader

PN - 90-0351

SN - A07790

Bottom:-

Model - Scanner 3000

PN - 00-0073R

SN - 50202631

Powers on.

Affymetrix GeneChip Fluidics Station

Model - 400

PN - 800101

SN - FS1134

Powers on.

Affymetrix GeneChip Hybridization Oven

Model - 645

PN - 00-0331

SN - C0105100

Powers on with rotation speed up to 80rpm. The oven is in good working order.

Affymetrix GeneChip Hybridization Oven

Model - 640

SN - OVN 021550

Powers on with rotation speed running up to 85rpm. The oven is in good working order.

Chemagen Chemagic Separation Module I with Software & Accessories

This was removed from a university lab where it was surplus to requirements.

Included in the system are;

1 x Chemagic Magnetic Separation Module I

10 x Tube Racks :-

7 hold 12 x 50ml tubes

2 Hold 12 x 15ml tubes

1 holds 12 x 0.5ml tubes

1 x Chemagen Biopolymer

Vulkan Technic

Order no. 200 03 014

The unit powers on but not tested further.

1 x Compaq PC

Model - Presario S0000

Chemagen/Chemagic Software MSM (Release 5.1.0.6) Installed

1 x DGM Monitor

Model - L-1731W

1 x Compaq Keyboard

1 x IBM Mouse

All PC equipment powers up and seems to be in full working order.

Boxed Accessories:-

178 x Genetix Lids (opened)

100 x Sealed 96 well 2.2ml storage plates

6 x Sealed Nunclon Surface plates

10 x Rubber plates

5 x 384 well plates (S300RXFL)

9 x 96 well 0.1ml slots

8 x 100mm Tissue Culture Dishes

11 x Chemagen Tubes (PK-400500-036-080403)

18 x Microcon YM-100 Regenerated Cellulose

38 x 2ml vials

17 Packs of MicroAmp Optical Caps (8 caps per strip, 12 strips per pack)

25 x Thermo Scientific 0.2ml Skirtled 96 well PCR Plates

57 x fisherbrand Dispenser Tips 12.5ml

Promega SV96 Total RNA Isolation System

2 x Denletstor Metal racks size 38.5 x 15.5cm, 160 squares 1.5 x 15cm each

2 x Wash Buffer 6 Bottles (both full) Volume unknown.

Promega Vac-Man Laboratory Vacuum Manifold

This unit was removed from a lab as surplus to requirements. The lab assured us it was in working order when last used, but we do not have a PC or software to test this instrument at our site.

In the testing we were able to carry out, the item powers on, both front lights were flashing orange. The microplate drawer also opened but we were unable to test it any further.

Item is in a good used cosmetic condition.

This Applied Biosystems 7300 Real Time PCR System was removed from a university lab where it was surplus to requirements.

The system is comprised of;

1 x Applied Biosystems 7300 Real-Time PCR Unit.

1 x Dell Optiplex 380 with Monitor, keyboard, mouse, USB cable & monitor cable.

Running Applied Biosystems Sequence Detection Software (SDS)

Version 1.2.3.

The Dell has a Fresh install of Windows XP.

The 7300 has had a function test run:

USB - Pass

CCD - Pass (version 2.19)

Filter Wheel - Pass (FW version G2.10)

Shutter - Pass (FW version G2.10)

Lamp - Pass (FW version G2.10)

Thermal Cycler - Pass (G2.10 Firmware is up to date)

The lamp status is Good.

It has 1444 Usage hours.

It was last replaced 08/01/2008.

The unit will require the ROI calibration.

This Qiagen QIAcube Nucleic Acid Purification System was removed from a university lab where it was surplus to requirements.

The unit powered up and we were able to run some pre-set programs. It ran these tests well. The lab has confirmed that it was functional prior to removal.

We ran a centrifuge program for 3 minutes which ran smoothly and we ran a shaker program for 1 minute which ran smoothly.

It is in good cosmetic condition with minor marks from use.

It comes with;

4 Boxes Filter Tips 1000ul (1024)

10 Boxes Rotor Adaptors (10 x 24)

4 Boxes Filter Tips 1000ul Wide bore (1024)

This Qiagen PyroMark Q24 Sequencer System was removed from a university lab where it was surplus to requirements.

The lab assured us this unit was working, but as we understand it requires two USB sticks from Qiagen, which unfortunately the lab did not provide to us and have been unable to find. This lack of USB sticks explains the below issue. We have not yet got a quote on the USB sticks from Qiagen, but we are open to negotiation on price of this unit depending on the costs of getting these sticks to run the unit.

The unit powers up with the power supplies provided but we were unable to run it as it required a 'USB memory'. A message appeared on the screen saying

'Please insert USB memory' when pressing RUN, another message appears 'trying to access USB memory'. We assume it requires external software on a USB which is not included.

Versions:

Revision: E

Software 1.46901

Hardware 0.3.20

The unit has a 24-well block

Dimensions:

Dia 2.6ml x 4.8ml Dep

It is in good cosmetic condition with some minor marks from use.

Included with it are;

1 x XP Power supply

Model No. PCM80PS24 2547

The unit powers on and is in good cosmetic condition.

1 x XP Power supply

Model No. PCM80PS12

The unit powers on and is in good cosmetic condition.

1 x Qiagen PyroMark Workstation

Model PyroMark Q24 MDx

This Agilent Stratagene Mx3005P Real-Time PCR System was removed from a university lab where it was surplus to requirements.

The main unit is in reasonable used cosmetic condition but it does have some scrapes, scratches and marks from use.

The unit powers up and both Green LED's light. You can also feel the well block heating up. The well block is 96 hole.

The system includes:

1 x Agilent Stratagene MX3005P. (various marks from use)

1 x Dell E8400 PC with keyboard & mouse which we believe to be brand new.

1 x iiyama monitor. (various marks from use)

The PC has has a new HDD fitted and is running a fresh install of Windows 7. Also installed is a copy of Stratagene MxPro - Mx3005P v4.10 build 389. The software will control the Mx3005P unit. We have run a basic heat cycle with it and varified that the lamp will turn on & off. It has not been tested any further.

The PC comes with a brand new sealed keyboard & mouse and a used iiyama monitor is also included.

The system should include everything required to operate.

This Applied Biosystems 7900HT Fast Real Time PCR System was removed from a university lab where it was surplus to requirements.

The system is in good used cosmetic condition.

We have powered the unit up and have been able to connect the unit to the PC but the software shows the serial number is invalid. It requires the 9 digit serial number from the rear of the main unit but it will only allow you to enter 6 digits!

We have spoken to Thermo who owns Applied Bioscience and they contacted a service engineer for us. The engineer said the issue can only be fixed with the engineer service tools, which could be done on-site post-purchase of this system.

It consists of the following parts:

1 x Applied Biosystems 7900HT

Part no. 4330966

SN - 279002990

1 x SICK CLV422-1910S02 Fixed barcode scanner

1 x AB 7067/4 Microplate stacker.

1 x Symbol Barcode Scanner.

1 x Dell Optiplex 960 PC with Dell Monitor, keyboard and mouse.

The has the following software installed:-

Applied Biosystems 7900HT Version 2.4 Sequence Detection Systems (SDS)

Applied Biosystems RQ Manager Version 1.2.1

We have all the passwords, which are available on request once the system has been sold.

This Analytik Jena AlphaSC Samplerblock Thermal Cycler was removed from a university lab where it was surplus to requirements.

The unit powers up and runs.

It is in good cosmetic condition with marks from use.

48/R9-96/R4.5

340:720.14

This Fluidigm C1 Single Cell Auto Prep System was removed from a university la where it was surplus to requirements.

The unit powers up and the internal compressor is working. We were unable to test it fully without the standards set required.

It is in excellent cosmetic condition but has some minor marks from general use.

Thermo Hybaid MCA Melting Curve Analysis qPCR System

This was removed from a university lab where it was surplus to requirements.

Item is in reasonable cosmetic condition with signs of damage such as residue and surface damage.

The item powers on with the front display light and fan in working order but we were unable to test it further.

Thermo Hybaid MCA Melting Curve Analysis qPCR System

This was removed from a university lab where it was surplus to requirements.

Item is in reasonable cosmetic condition with signs of damage such as residue and surface damage.

The item powers on with the front display light and fan in working order but we were unable to test it further.